Over the course of billions of years, biological evolution has created and refined numerous elegant processes to build multifunctional structures that suit specific structural, mechanical and physiologic demands. Modern techniques, however, are unable to build complex in vivo like functional structures using biological materials. Our goal is to develop new processing and printing technologies to create reliable models from single cell to tissue scale to capture key aspects of in vivo physiology. With multidisciplinary expertise in mechanical engineering, laser optics, biomaterials and cell biology, we have developed a biomanufacturing toolbox that can be broadly applied in the fields of single cell biology, developmental biology, disease modeling, and tissue regeneration.